Figure 3  .

Accurate Genescan sizing of injected and uninjected PS-ODN using oligonucleotide size standards. (A) The original 20-mer DS 012 was compared directly with a series of PS-ODNs varying in size from 15 to 25 nucleotides in length and their electrophoretograms superimposed using the internal standard as a reference. Oligonucleotides within this range of size were clearly resolved using the Genescan analysis software under the altered run conditions. (B) DNA extracted from the retina of an eye 12 weeks following injection with DS 012 for 12 weeks was run and compared directly with the oligonucleotide size standard. The extracted oligonucleotide demonstrated some minor peaks representing N-1 degradation products.