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Detection of herpes simplex virus DNA in tear samples. (Top) Agarose gel electrophoresis of PCR products. Lanes 1-17 represent tears from 17 eyes of atypical epithelial keratitis patients. P=positive control; N=negative control. Patient Nos 3, 5, 6, 7, 8, 10, 11, 17 showed 142 bp amplified bands. (Bottom) Southern blot hybridisation confirms results of agarose gel electrophoresis. Lanes 1-P are same as those at the top.
