Figure 3.

Metabotrobic glutamate receptor activation of GluR2–cMyc protein translation. A, C, E, and G are phase contrast photographs of cell fields that correspond to B, D, F, and H, respectively. (B) These cells show that transfection of GluR2–cMyc fusion construct mRNA into cells, in the absence of DHPG stimulation, results in minimal translated protein. (D) This nontransfected dendritic process is stained with the cMyc antibody and shows no immunoreactivity. (F) This nontransfected dendrite is stained with an ATF-2 antibody and exhibits no immunostaining. The Inset in the upper left corner shows a stained neuron from the same culture, thus showing that ATF-2 is present in the neuronal cell body but is absent from dendrites. (H) GluR2–cMyc immunoreactivity generated after transfection of the GluR2–cMyc fusion construct mRNA that had been stimulated by the addition of 500 μM DHPG into the media. Arrow points to the individual isolated dendrite described herein.