Figure 1.

GABARAP cluster GABA_A receptors. This figure shows immunofluorescence of GABA_A receptor expressed in QT-6 cells with or without GABARAP. All of the cells shown here expressed α1β2γ2L GABA_A receptor and stained with antibody bd17. (A) Large flat cell expressing GABARAP gives clustered GABA receptors. (B) Spherical cell with GABARAP gives clusters; note “broken” periphery. (C) Large flat cell with antisense GABARAP shows diffuse GABA_A receptors. (D and E) Confocal images of antibody-labeled GABA_A receptors (D, green) and GABARAP (E, red) at the cell surface. (F) A merge of D and E, showing colocalization in yellow.