Figure 3.

Specific immunoprecipitation of the farnesylated form of RhoB in Sf9 cells. (A) Five micrograms of delipidated proteins from Sf9 cells that had expressed the different prenylated forms of RhoB was immunoprecipitated by using 0.5 μl of the anti-F-Cys Ab and analyzed by Western blotting with a rabbit anti-RhoB. Five micrograms of Sf9 cell lysates was analyzed by Western blot using the rabbit anti-RhoB Ab (Bottom). (B) An amount of 2.5 μl of the anti-F-Cys Ab was preincubated with 1 ml of BSA or F-Cys-BSA coupled to agar beads. The supernatant was recovered and used to immunoprecipitate RhoB from RhoB-CAIM expressing Sf9 cells, as described in A.