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. 2000 Oct 10;97(21):11626–11631. doi: 10.1073/pnas.97.21.11626

Figure 4.

Figure 4

Specific immunoprecipitation of the farnesylated form of RhoB in NIH 3T3 and COS-7 cells. (A) Seventy-five micrograms of proteins of NIH 3T3 cells transfected by the different prenylated forms of RhoB was immunoprecipitated with 1 μl of the anti-F-Cys Ab and analyzed by Western blot probed with a rabbit anti-RhoB Ab. (B) One milligram of delipidated membrane proteins of COS-7 cells was immunoprecipitated with nonimmune rabbit IgG (ni IgG) or anti-F-Cys Ab and analyzed by Western blot probed with a rabbit RhoB Ab. (C and D) Ten micrograms of NIH 3T3 lysates or COS-7 membranes, respectively, was analyzed by Western blot using the rabbit anti-RhoB Ab.