Skip to main content
. 2000 Sep 26;97(21):11632–11637. doi: 10.1073/pnas.190309197

Figure 2.

Figure 2

Isolation of cDNAs encoding ABRE-binding proteins by the yeast one-hybrid system. (A) Confirmation of five isolated cDNAs encoding ABRE-binding proteins by the yeast one-hybrid system. Five plasmids containing insert DNA from AREB1, AREB2, AREB3, GBF1, and GBF3 were retransformed into yeast strains carrying the reporter genes HIS3 and lacZ under the control of the 77-bp fragment containing two ABREs. The transformants were examined for growth in the presence of 3-AT and β-galactosidase (β-gal) activity. (B) Binding specificity of proteins encoded by isolated cDNAs. Five plasmids were used for transformation into yeast carrying the reporter genes under the control of the 77-bp fragment containing mutated ABREs. (C) Activation of reporter genes in yeast by proteins encoded by isolated cDNAs. The insert DNA fragments of the five isolated cDNA clones were cloned into the yeast expression vector YepGAP and used for transformation into yeast carrying the reporter genes under the control of the 77-bp fragment containing two ABREs.