Table 1.

The cell-killing activities of EGL-1 and EGL-1 mutants

hsp construct*	Array	egl-1(n3082)		ced-9(n1950)
		No. of embryos	No. of corpses†	No. of embryos	No. of corpses†
None		30	0.2  ± 0.4	30	3.3  ± 1.8
Vector	1	30	0.3  ± 0.5	30	1.6  ± 1.6
	2	30	0.2  ± 0.5	30	2.7  ± 1.7
	3	30	0.3  ± 0.5	30	2.6  ± 1.3
EGL-1	1	31	46.0  ± 24.6	30	4.0  ± 1.9
	2	31	33.6  ± 20.9	30	3.8  ± 1.8
	3	30	40.5  ± 15.7	30	2.3  ± 1.8
EGL-1(D63R)	1	32	43.4  ± 24.3	31	2.6  ± 2.3
	2	30	31.9  ± 25.1	30	2.8  ± 2.7
	3	30	46.9  ± 13.2	30	3.2  ± 2.0
EGL-1(D64RD66R)	1	30	0.6  ± 1.3	30	3.2  ± 2.6
	2	33	0.2  ± 0.6	32	2.6  ± 1.5
	3	31	0.7  ± 1.3	30	3.4  ± 1.8
EGL-1(D63ND64ND66N)	1	30	34.8  ± 17.0	30	3.6  ± 2.5
	2	30	32.2  ± 19.0	30	2.6  ± 1.5
	3	30	35.0  ± 17.6	30	3.4  ± 1.9

^*The hsp constructs (at 50 μg/ml each) were injected into ced-1(e1735); egl-1(n1084 n3082) unc-76(e911) animals with pTG96 (at 20 μg/ml), which expresses GFP in many somatic cells in most of the developmental stages (35), and p76-16B (at 50 μg/ml), which rescues unc-76 uncordinated phenotype (36). The transgenes were then crossed into ced-1(e1735); ced-9(n1950) mutant background. 

^† Heat shock experiments and cell-killing assays were performed as described (7). Fluorescent transgenic embryos at the three-fold or later stages of embryogenesis were scored for cell corpses in the head region. Data shown are means ± SEM. All data except the first row depicts results obtained after heat-shock treatment. For comparison, ced-1(e1375) animals have an average of about 31 cell corpses in the same region.