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Effect of TNF-α (10 ng/ml) on aminopyrine accumulation by parietal cells in the basal state and when stimulated by histamine (His; 10−4 M), forskolin (Fsk; 10−5 M), dibutyryl-cAMP (dbcAMP; 10−4 M), carbachol (Cbh; 10−4 M), gastrin-17 (Gas; 10−7 M), or A23187 (10−6 M). Cells were exposed to TNF-α for 15 minutes before stimulation. Results are expressed as percentage of basal aminopyrine uptake in the absence of cytokine (mean (SEM)), n=5-6. *p<0.05; **p<0.01.
