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HLA-DR molecule expression on the surface of untreated intestinal HT29-19A cells, and cells treated on their basal side with 100 U/ml recombinant human interferon γ (IFNγ) for 48 or 72 hours. A monoclonal antibody recognising HLA-DR (clone L243) was used. Untreated cells did not express HLA-DR. After 48 hours of IFNγ treatment, HLA-DR expression was significant, and after 72 hours, it had greatly increased. A positive control was run with EBV-B cells.
