Figure 2 .

(A) Representative behaviour of liver cytosol antibody type 1 (LC1) concentration and serum alanine aminotransferase (ALT) levels during immunosuppression in patient 19. LC1 immunoreactivity against the 58 kDa (kD) human liver cytosolic (HLC) polypeptide was evaluated by immunoblotting visualised by enhanced chemiluminescence and quantified by densitometric analysis. A clear correlation was observed between LC1 concentration and ALT levels. (B) Independent behaviour of LC1 with respect to liver/kidney microsomal antibody type 1 (LKM1) in patient 13. LKM1 reactivity against the 50 kDa human liver microsomal (HLM) protein—that is, CYP2D6—remained essentially unchanged. An additional 52 kDa band, corresponding to residual human IgG both in microsomal and cytosolic fractions, is also evident.