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. 1998 Sep;43(3):365–374. doi: 10.1136/gut.43.3.365

Figure 4 .

Figure 4

High molecular weight kininogen cleavage in Lewis rats treated with indomethacin shown by western blotting using anti-high molecular weight kininogen antibodies. Lane 1 shows pooled non-activated normal rat plasma (N), and lane 2 pooled rat plasma following exposure to kaolin (K). Lanes 3-5 represent the plasma samples of three control rats, while lanes 6-8 are plasma samples of rats 14 days after treatment with indomethacin. The 120 kDa band represents HK and is visualised across lanes 1 and 3-8, but is absent in lane 2. The broad band localised at about 68 kDa across all lanes indicates cross reacting T kininogen (TK) and albumin. The band situated between albumin and the 43 kDa standard, visualised on lane 2 and lanes 6-8, but not on lane 1 and lanes 3-5, represents the heavy chain of high molecular weight kininogen (HC-HK, 62 kDa). The delicate band situated just above 32 kDa, standard across all lanes, represents a non-specific reaction with alkaline phosphatase that hydrolyses the BCIP/NBT substrate used to develop the colour.