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. 2000 Oct 17;97(22):11936–11941. doi: 10.1073/pnas.220252297

Figure 2.

Figure 2

Phosphorylation of mouse p53 at Ser-18 in ES cells at various times after IR or UV treatment. ES cells were exposed to 5 Gy IR or 60 J/M2 UV light. Cell extracts from irradiated cells and untreated controls were analyzed by Western blotting with antibodies specific for p53 or p53 phosphorylated at Ser-18. The phosphorylated p53 (Top) and total p53 are indicated. Time after irradiation is indicated at the top of each lane.