Figure 3 .

Competition assays of the activated NF-κB. Peritoneal macrophages were collected 12 hours after the induction of taurocholate pancreatitis. Nuclear extracts were preincubated with no reagents (lane 1), a 10-fold (lane 2) or a 100-fold (lane 3) molar excess of unlabelled κB oligonucleotide, or a 100-fold molar excess of mutant κB oligonucleotide (lane 4). In the competition assay, an excess of the oligonucleotide competitor was preincubated with the nuclear extract for 60 minutes at room temperature.