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GC profiles of the hydroxylated products of (−)-limonene generated by wild-type (wt) cytochrome P450 SM12 and PM2, and mutants SM12 F363I and PM2 I364F, when reconstituted with purified spearmint NADPH:cytochrome P450 reductase. The products identified are (−)-trans-carveol (16.53 min) and (−)-trans-isopiperitenol (15.56 ± 0.01 min).
