Table 1.

Schematic representation of the hydroxylase chimeras and their expression levels and activities  

Chimeric cDNAs are represented by combination of white and gray boxes representing the wild-type SM12 (white) and PM2 (gray) coding sequences. The positions of the restriction sites are indicated by arrows. For the chimeras constructed by PCR, breaks have been introduced in the presentation to expand the region of interest. Expression levels were determined from the CO-difference spectra, and activity levels are relative to wild-type PM2 activity.