Table 2.
Point mutants of the hydroxylases and their expression levels and activities
| Expression level, pmol P450/mg protein | Limonene hydroxylase activity, relative units
|
||
|---|---|---|---|
| C3 | C6 | ||
| SM12 wild type | 520 | 0 | 52.0 ± 5.4 |
| PM2 wild type | 1470 | 100 | 0 |
| SM12 L357M | 328 | 0 | 31.0 ± 0.4 |
| SM12 L359M | 613 | 0 | 42.3 ± 5.5 |
| SM12 F363I | 460 | 100 ± 15 | 0 |
| SM12 Q369S | 270 | 0 | 49.5 ± 1.6 |
| SM12 S370C | 614 | 0 | 56 ± 12 |
| PM2 M358L | 1090 | 100 ± 18 | 0 |
| PM2 M360L | 1300 | 7.5 ± 7.2 | 0 |
| PM2 I364F | 375 | 0 | 0 |
| PM2 S370Q | 884 | 98.2 ± 2.5 | 0 |
| PM2 C371S | 410 | 86.9 ± 24.0 | 0 |
Expression levels were determined from CO-difference spectra, and activity levels are relative to wild-type PM2 activity.