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Cleavage of Na,K-ATPase in conditions permitting phosphorylation. The enzyme was suspended in the medium containing 130 mM NaCl or other salts, and incubated with 5 mM ascorbate/H2O2, 5 μM FeSO4 as follows: (A) 166 μM ATP and indicated times; (B) 40 μM AMP-PNP and indicated times; (C) 500 μM ATP, 10 min incubation; (D) 500 μM ATP, oligomycin, 200 μg/ml, 5 min incubation; (E) 20 μM AMP-PNP, 300 mM choline chloride or 300 mM NaCl, 5 min incubation.
