Figure 3 .
Cryostat sections from rat, sham operated (A and D), one week (B and E), and 10 weeks (C and F) bypassed ileum, immunostained for VIP (A-C) and nNOS (D-F). VIP immunoreactive nerve fibres are numerous in mucosa/submucosa, muscular layers, and enteric ganglia at all time points studied. nNOS immunoreactive nerve fibres are restricted to the muscle layers and myenteric ganglia. The number of nNOS immunoreactive cell bodies within myenteric ganglia (indicated by arrows) is increased in bypassed ileum, particularly 10 weeks postoperatively (F). Original magnification × 130.