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Potentiation of cytomix induced iNOS activity by forskolin (FSK) and Db-cAMP in DLD-1 cells. Cells were incubated in serum free medium with cytomix alone (white bars) or FSK, with or without 3-isobutyl-1-methylxanthine (IBMX) or Db-cAMP. Results are expressed as percentage of induction with cytomix alone and represent means (SEM) from at least three different experiments, each done in triplicate. *p<0.05, **p<0.01, ***p<0.001 compared with value obtained in cytomix treated cells.
