Figure 4  .

Substance P receptors in hepatocellular carcinoma (HCC) (A-C) and a hepatic adenoma (D-F). (A,D) Haematoxylin and eosin stained sections. In A, the tumour (T) is surrounded by stroma containing peritumorous vessels (PTV). In D, the adenoma contains arteries (arrowheads) and veins (arrows). (B,E) Autoradiograms showing total binding of ¹²⁵I- BHSP (Bolton-Hunter substance P). (C,F) Autoradiograms showing non-specific binding of ¹²⁵I- BHSP (in the presence of 10⁻⁶ M substance P). Peritumorous vessels are strongly labelled in B. Intratumorous arteries are strongly labelled in E, whereas veins are not. In both cases, the tumours are substance P receptor negative. Bars = 1 mm.