Figure 1  .

Analysis of keratinocyte growth factor (KGF), CD45, and α2(I) type I procollagen transcripts in lamina propria lymphocytes (LPL) and intraepithelial lymphocytes (IEL) isolated from uninvolved and inflamed inflammatory bowel disease tissues. RNAs (2 µg samples) were hybridised to ³²P-labelled antisense probes and then digested with RNAse. Protected hybrids were resolved by electrophoresis through denaturing polyacrylamide gels. RNAs extracted from the embryonic lung fibroblast cell line, M426, and from three primary cultures of human intestinal fibroblasts isolated from normal (N), ulcerative colitis (UC), and Crohn's disease (CD) tissue, were included as positive controls for KGF and α2(I) type I procollagen expression. RNA isolated from the MOLT-4 T cell line was used as a positive control for CD45 expression. Total RNAs isolated from an uninvolved (U) and inflamed (I) region of ulcerative colitis were included for comparison. Exposure times were as follows: KGF, 72 h; CD45, 72 h; α2(I) type I procollagen, 24 h.