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(A) Bilateral Fos-like immunoreactivity (Fos-LI) in a section of L6-S1 spinal cord following repeated (2 h, 80 mm Hg for 30 s every 120 s) colorectal distention. (B) Areas used to count regional Fos-LI: area 1, superficial laminae I and II; area 2, laminae III and IV; area 3, lateral laminae V-VII (intermediolateral horn) and lateral ventral horn; area 4, medial laminae V-VII and X, and medial ventral horn.
