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. 2000 Oct 10;97(22):11972–11977. doi: 10.1073/pnas.220326097

Figure 4.

Figure 4

Immunodepletion and rescue. (A) Mitotic extracts were immunodepleted with control IgG (lane 1), with a mixture of anti-XCAP-C, -D2, -E, and -G (lanes 2–5), or with a mixture of anti-XCAP-D2 and -G (lanes 6 and 7). Then a purified fraction of 13SC (lane 3), 8SC (lane 4), 11SR (lanes 5 and 7), or buffer (lanes 2 and 6) was added back into the depleted extracts. Aliquots of each extract were analyzed by immunoblotting. (B) After incubating sperm chromatin with each extract at 22°C for 2 h, chromatin was isolated and bound proteins were analyzed by immunoblotting. (C) Sperm chromatin was incubated with each extract (1–7 as in A) at 22°C for 2 h, fixed, and stained with 4′,6-diamidino-2-phenylindole. (Bar, 10 μm.)