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Calcium-dependent IgE recognition of purified carp parvalbumin pI 4.7. Nitrocellulose-blotted carp parvalbumin pI 4.7 was exposed to serum IgE from four patients allergic to fish (Nos 1, 2, 3, and 4) and to a mouse monoclonal anti-parvalbumin antibody (mαparv.) in the presence (+) and absence (−) of calcium.
