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. 2000 Jul;47(1):63–73. doi: 10.1136/gut.47.1.63

Figure 1  .

Figure 1  

Evaluation of equal amplification efficiencies of the MMP-1 PCR product and the MMP-1 competitor construct. Purified MMP-1 PCR product (+; 247 bp) and the MMP-1 competitor construct (#; 222 bp) were mixed in an OD ratio of 1:1 (lane 1) and 1:5 (lane 6). These solutions were diluted 1:200 000 and used as templates for PCR for 27 cycles (lanes 2, 7) and 35 cycles (lanes 3, 8). An inverted image of an ethidium bromide stained gel is shown demonstrating equal density ratios before (lanes 1, 6) and after (lanes 2, 3, 7, 8) reamplification. Lanes 4 and 5 contain a DNA mass standard used for calibration of the densitometry. Numbers on the right indicate the DNA size in base pairs (bp).