Figure 1  .

Differential chemokine expression in CaCO-2 cells. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to determine monocyte chemoattracting protein 1 (MCP-1) (A) and interleukin (IL)-8 (B) mRNA expression in CaCO-2 cells on stimulation of CaCO-2/leucocyte co-cultures or CaCO-2 cells alone with non-pathogenic E coli, L johnsonii, and L sakei (16 hours, 10⁶ and 10⁷ cfu/ml), respectively. Lipopolysaccharide (LPS, 1 µg/ml), IL-1β (10 ng/ml) and culture medium (no treatment) were used as controls. Results represent one of three independent experiments.