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Figure 3  .

Figure 3  

Polarised secretion of tumour necrosis factor alpha (TNF-α) and interleukin (IL)-1β by leucocyte sensitised CaCO-2 cells. Stimulation of CaCO-2/leucocyte co-cultures or CaCO-2 cells alone with non-pathogenic E coli (107 cfu/ml). After 12 hours of incubation, CaCO-2 cells were transferred to fresh culture medium. After an additional 24 hours of incubation, secretion of TNF-α and IL-1β by leucocyte sensitised CaCO-2 cells was determined in the apical and basolateral compartments (transfer experiment). Controls (no treatment) did not induce cytokine secretion. Data are mean (SD) of triplicate values and represent one of three independent experiments.