Figure 1  .

Cytotoxicity assay of an individual with acute hepatitis B. (A) Peripheral blood mononuclear cells were stimulated with the HBc/HLA B*0702 dual transfectant and then tested against HBc/B*0702 dual transfectant, vector/B*0702 dual transfectant, or L721.221/HBc single transfectant. Cold targets were used at a ratio of 10:1 to hot targets. (B) To confirm the presence of a major histocompatibility complex (MHC) class I restricted cytotoxic response, the culture was restimulated with the dual transfectant line and retested in the presence of the anti-MHC class I antibody W6/32, an isotype matched control antibody, or no antibody.