Figure 4  .

Immunohistochemical staining for collagenase-3 (A, D) and MT1-MMP (B, E) in human oesophageal carcinoma. (A) Collagenese-3 was strongly expressed in tumour cells, especially at the invasive front of the tumour cells. Also, weak expression was detected in stromal fibroblasts surrounding the tumour cells. (B) In a parallel section, MT1-MMP was detected mainly in tumour cells and weakly expressed in stromal fibroblasts surrounding the tumour cells. Also, MT1-MMP was detected in the same collagenase-3 positive tumour cells. (C) Haematoxylin-eosin staining in a parallel section. (D, E) Neither collagenase-3 nor MT1-MMP was expressed in normal tissues. Original magnification ×100.