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. 2000 Oct 17;97(22):11978–11983. doi: 10.1073/pnas.210392297

Figure 5.

Figure 5

A time course of the primary, second primary, and 5-nt cuts using the quench-flow machine. The same substrates were used as in Fig. 4. A ratio of 2 enzymes to 1 substrate was used. The time was measured in milliseconds and the time points are labeled above each lane. (A) The time course of the 3′ end-labeled 41-nt RNA. The 23-nt and 5-nt products are the primary and 5-nt cuts, respectively. (B) The time course with the 50-nt RNA substrate. The 32-nt and 5-nt products are the primary and 5-nt cuts.