Developmental pattern of sucrase-isomaltase (SI) mRNA expression. In situ hybridisation was performed on sections of the proximal jejunum using an SI antisense cRNA probe as described in materials and methods. Dark field (A, C, D) and bright field illuminations (B, E-H) of intestinal sections from 11 (A, B), 16 (C), 18 (D), 21 (E), and 60 (F-H) day old rats. Controls: hybridisation with a sense probe (G) or treatment of the section with RNAse before hybridisation (H). Magnifications: A, C-F ×50; B, G, H, ×250.