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Detection of leptin mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Lanes 1+2, adipose tissue; lanes 3+4, human gastric mucosal biopsy; lanes 5+6, primary cultured human gastric epithelial cells; lanes 7+8, human cancer cell line AGS. The PCR products of leptin specific mRNA are demonstrated in lanes 2, 4, 6, and 8. Lanes 1, 3, 5, and 7 show the house keeping gene GAPDH. M, size marker 100 bp DNA ladder.
