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Detection of different leptin receptor mRNA splice variants by reverse transcriptase-polymerase chain reaction (RT-PCR). (A) Human gastric mucosal biopsy. (B) Primary cultured human gastric epithelial cells. (C) Human cancer cell line AGS. huOB-R, human long leptin receptor isoform; huB219.1-huB219.3, human short leptin receptor isoforms, huOB-Runi, primers chosen to detect all receptor isoforms.
