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Binding for endothelin receptors (ETA and ETB) on LIM1215 (left) and HT29 (right) cell cytospins was demonstrated by autoradiography. Slides were incubated with ETA antagonist (125I PD151242) or ETB agonist (125I BQ3020) for total binding. Non-specific binding was determined by incubation with excess unlabelled ligand. Total binding was clearly in excess of non-specific binding, suggesting the presence of both receptors in these cell types.
