Figure 6  .

Hypoxia increased vascular endothelial growth factor (VEGF) mRNA half life in HepG2 cells. Cells were incubated for 16 hours under normoxic or hypoxic conditions and subsequently incubated with 150 µM DRB to block transcription. Cells were harvested for RNA after 0, 30, 60, 120, 180, 240, and 360 minutes. VEGF mRNA was then determined by quantitative competitive reverse transcription-polymerase chain reaction as described in materials and methods. Mean (SEM) values of three independent experiments.