Figure 10  .

Immunohistochemical localisation of P-selectin in the colon. Staining of colon sections obtained from sham operated rats with anti-intercellular adhesion molecule 1 (ICAM-1) antibody showed specific staining along vessels, demonstrating that ICAM-1 is constitutively expressed (A). Ileum sections from sham operated rats revealed no positive staining for P-selectin (B). Sections obtained from dinitrobenzene sulphonic acid (DNBS) treated rats showed intense positive staining for ICAM-1 (D) and P-selectin (E) on endothelial cells. The degree of endothelial staining for ICAM-1 (G) and P-selectin (H) was markedly reduced in tissue sections obtained from calpain inhibitor I treated rats. (C), (F), and (I) represent the staining combination of (A-B), (D-E), and (G-H) respectively. Original magnification ×145. The figure is representative of at least three experiments performed on different experimental days.