Figure 8  .

Immunohistochemical localisation of nitrotyrosine and poly (ADP-ribose) polymerase (PARP) in the colon. No positive staining for nitrotyrosine (A) or PARP (B) was found in colon sections from sham operated rats. Immunohistochemical staining for nitrotyrosine (D) and PARP (E) showed positive staining localised in the injured area from a dinitrobenzene sulphonic acid (DNBS) treated rat. The intensity of the staining for nitrotyrosine (G) and PARP (H) was significantly reduced in the ileum from calpain inhibitor I treated rats. (C), (F), and (I) represent the staining combination of (A-B), (D-E), and (G-H), respectively. Original magnification ×145. The figure is representative of at least three experiments performed on different experimental days.