Figure 1  .

Detection of TFF2 protein in gastric juice over 24 hours. (A) Modified sequence of the mature TFF2 protein. The positions of the two trefoil domains are indicated, as are the amino acids included in each of the three loops formed during folding of the trefoil domains. The single N-glycosylation recognition sequence, which is a potential glycosylation site located in loop 1 of the first trefoil domain, is boxed. The lysine residue (K) which replaces the asparagine residue found in the previously published sequence¹ at position 99 is also boxed. The 16 amino acid peptide synthesised for use as an immunogen is also indicated. Spaces have been inserted in the sequence after residues 6, 49, and 98 to indicate the limits of the trefoil domains. (B) Human gastric juice was collected at two hourly intervals over a 24 hour time course from a young volunteer. Aliquots were subjected to electrophoresis on a denaturing polyacrylamide gel, transferred to PVDF membranes, and reacted with TFF2 antisera as described in the methods section. Downward pointing arrows indicate meal times. The positions of glycosylated TFF2 (Glyc. TFF2) and non-glycosylated TFF2 (TFF2) are shown on the right hand side.