Figure 2  .

Expression of SOX10 in the aganglionic (A), hypoganglionic (H), and normoganglionic (N) colon segments of a Hirschsprung's disease (HD) patient examined by reverse transcription-polymerase chain reaction (RT-PCR) in the presence (+) and absence (−) of reverse transcriptase. Expression of SOX10 in normal control colon tissue (C) is also shown. The expected SOX10 fragment of about 250 bp was amplified from normal control and HD patient samples. As a control the β-actin band of about 300 bp was also amplified from all samples. No DNA fragment could be amplified from cDNA samples that lacked reverse transcriptase (−) indicating that the amplified bands were derived from mRNA.