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Southern analysis of putatively clonal isolates derived from eight parent strains (lanes 1-8) that were originally iceA1+/2+ by polymerase chain reaction. Four were iceA1+, three iceA2+, and one had no hybridisation. Positive controls (lanes 9 and 10) were 26695 (iceA1+) and J99 (iceA2+).
