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Original tracings showing dose dependent increases in human colon tonic contractions by anti-M2 peptide IgG (IgG log [M]) from chagasic patients with megacolon alone (A) and after exposed tissues to 5×10−6 M AF-DX 116 (B), 5×10−5 M synthetic M2 peptide (C), and 5×10−6 M pertussis toxin (D) over 30 minutes before anti-M2 peptide IgG from chagasic patients with megacolon was added.
