Figure 1 .
Effect of interleukin 2 (IL-2) on cAMP dependent chloride secretion in BN cells. (A) Representative traces of control (Con) and IL-2 treated monolayers. (B) Representative traces of control and IL-2 treated monolayers in chloride free buffer. (C) Secretagogue stimulated short circuit current (ΔIsc) in normal buffer and paired monolayers in chloride free buffer (n=7-9 pairs, data are mean (SEM)). (D) Constitutive expression of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA in BN cells and controls (human small intestinal biopsy). Cells depicted in (A-C) were treated with IL-2 5 U/ml for 24 hours prior to the study; secretion, stimulated with 3-isobutyl-1-methyl xanthine (IBMX) 3×10−4 M and forskolin 10−6 M (arrows). bp, base pairs. **p<0.01 compared with control and †p<0.02 compared with respective control period by paired t test.