Figure 4  .

Reactivity with oxidised cardiolipin or human serum albumin (HSA) complexed with lipid peroxidation products following preabsorption with the β₂ glycoprotein 1 (β₂-GP1) of sera from alcoholic patients. Fifteen sera from patients with alcoholic liver disease displaying high titres of IgG against both cardiolipin and β₂-GP1 were incubated overnight at 4°C on irradiated ELISA plates coated with β₂-GP1 and tested for IgG binding to oxidised cardiolipin (Ox-CL) or HSA modified by reaction with malondialdehyde (HSA-MDA) or products of arachidonic acid peroxidation (HSA-APP). Results are mean (SD) ELISA values obtained with native sera or β₂-GP1 preabsorbed sera. *p<0.0001 versus non-pre-absorbed sera.