Figure 3.

Expression of Bcl-2 blocks cytochrome c release and apoptosis but does not affect Bax translocation. (A) VM10-bcl cells are generated by stably transfecting a Bcl-2 expressing plasmid, and VM10-C is a cell line transfected by the control vector. The Western blot was probed with a monoclonal antibody against Bcl-2. (B) Bcl-2 blocks apoptosis in VM10 cells. The cells were shifted to 32°C for 24 h, and DNA was analyzed by agarose gel electrophoresis. (C) Bcl-2 expression blocks cytochrome c release but has no effect on Bax translocation. Extracts were prepared from mitochondria and cytosolic fraction of VM10-bcl and VM10-C cells at 32°C. The protein concentration was measured using a protein assay kit (Bio-Rad). An equal amount of protein was loaded onto the gel. Bcl-2, Bax, and cytochrome c proteins were detected by Western blotting. Cytosolic actin was used as a control.