Figure 3.

Thrombin stimulation of platelets from SLP-76-deficient and heterozygous mice results in comparable activation of αIIbβ3. Washed murine platelets were left unstimulated or stimulated with 0.5 unit/ml thrombin or 50 ng/ml convulxin for 10 min at 37°C. Cells then were incubated with 200 μg/ml FITC-fibrinogen and analyzed by flow cytometry, normalizing for the unstimulated controls. Results from three separate experiments are shown with error bars representing SD of the mean.