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E10R expression is required for the disulfide bond in the G4L protein. Cells were infected with vE10Ri or vA9Li or wild-type (WT) vaccinia virus in the presence or absence of 50 or 200 μM IPTG. Samples were alkylated with AMS and separated by SDS/PAGE, and the proteins were detected by Western blotting by using polyclonal anti-G4L antibody (10) followed by peroxidase-conjugated anti-HA antibody. Arrows indicate the reduced (red) and disulfide-bonded (ox) forms of the G4L protein.
