Figure 2  .

(A) Nested polymerase chain reaction (PCR) amplification of adenovirus, performed on left ventricular and septal tissue obtained from explanted hearts and biopsies taken from patients with familial early dilated cardiomyopathy. Representative data are shown in lanes 8-13. Other lanes are: molecular weight markers in the first and last lanes; culture grown adenovirus (lane 2), 10 and 5 copies of plasmid pJM17 carrying adenovirus sequences used for sensitivity assessments (lanes 3 and 4, respectively), uninfected mouse heart (lane 5), reagent contamination controls for first and second round of PCR amplification (lanes 6 and 7, respectively). (B) Nested PCR amplification of human cytomegalovirus (HCMV). The order of presentation of samples is as for panel (A). Culture grown HCMV is shown in lane 2. The sensitivity controls used were 100 and 10 copies of the plasmid pMV100 carrying HCMV sequences (lanes 3 and 4, respectively). (C) Amplification of the myoglobin gene from DNA. Cardiac samples are shown in wells 8 to 13. Adenovirus and HCMV cell cultures are shown in wells 2 and 3, respectively. Uninfected murine heart is shown in well 6 and the reagent negative control in well 7. No samples were loaded in wells 4 and 5.