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. 2000 Oct 17;97(22):12103–12108. doi: 10.1073/pnas.210394297

Figure 2.

Figure 2

Effects of IGF-1 stimulation on tyrosine phosphorylation of proteins that coimmunoprecipitate with E-cadherin. Cells were serum starved for 16 h and treated with recombinant 1.3 nM (10 ng/ml) IGF-1 for 10 min. Cells were lysed in Nonidet P-40 lysis buffer, and proteins were immunoprecipitated using anti-E-cadherin antibody (clone HECD-1). Immunoprecipitates were assessed for tyrosine phosphorylation (a) using phosphotyrosine antibody (PY99). Two proteins of approximate molecular mass of 180 kDa and 90 kDa were identified by Western blotting as insulin receptor substrate-1 (IRS-1) and β-catenin (b).