Figure 4.

Effects of IGF-1 stimulation on the localization of β-catenin in c10 cells. (a) Subconfluent c10 cells were serum starved for 16 h and were treated with 1.3 nM IGF-1 for 10 or 30 min. Cells were fractionated into 1% digitonin or RIPA-soluble fractions, and equal amounts of each soluble fraction were separated by SDS/PAGE and immunoblotted for β-catenin. (b) Subconfluent c10 cells were serum starved for 16 h and were treated with 1.3 nM IGF-1 or vehicle control for 30 min at 37°C. Cells were stained with an antibody for β-catenin that was visualized with a fluorescent secondary antibody. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI). (×400.)